Immunoreactivity characterisation of the three structural regions of the human coronavirus OC43 nucleocapsid protein by Western blot: Implications for the diagnosis of coronavirus infection
Identifieur interne : 001D07 ( Main/Exploration ); précédent : 001D06; suivant : 001D08Immunoreactivity characterisation of the three structural regions of the human coronavirus OC43 nucleocapsid protein by Western blot: Implications for the diagnosis of coronavirus infection
Auteurs : Fang-Ying Liang [Taïwan] ; Leng-Chieh Lin [Taïwan] ; Tsung-Ho Ying [Taïwan] ; Chen-Wen Yao [Taïwan] ; Tswen-Kei Tang [Taïwan] ; Yi-Wen Chen [Taïwan] ; Ming-Hon Hou [Taïwan]Source :
- Journal of Virological Methods [ 0166-0934 ] ; 2012.
Descripteurs français
- KwdFr :
- Adolescent, Adulte, Adulte d'âge moyen, Animaux, Anticorps antiviraux (sang), Coronavirus humain OC43 (immunologie), Femelle, Humains, Infections à coronavirus (diagnostic), Jeune adulte, Mâle, Protéines nucléocapside (immunologie), Protéines recombinantes (immunologie), Sujet âgé, Sujet âgé de 80 ans ou plus, Technique de Western (), Techniques de laboratoire clinique ().
- MESH :
- diagnostic : Infections à coronavirus.
- immunologie : Coronavirus humain OC43, Protéines nucléocapside, Protéines recombinantes.
- sang : Anticorps antiviraux.
- Adolescent, Adulte, Adulte d'âge moyen, Animaux, Femelle, Humains, Jeune adulte, Mâle, Sujet âgé, Sujet âgé de 80 ans ou plus, Technique de Western, Techniques de laboratoire clinique.
English descriptors
- KwdEn :
- Adolescent, Adult, Aged, Aged, 80 and over, Animals, Antibodies, Viral (blood), Blotting, Western (methods), Clinical Laboratory Techniques (methods), Coronavirus Infections (diagnosis), Coronavirus OC43, Human (immunology), Female, Humans, Male, Middle Aged, Nucleocapsid Proteins (immunology), Recombinant Proteins (immunology), Young Adult.
- MESH :
- chemical , blood : Antibodies, Viral.
- diagnosis : Coronavirus Infections.
- immunology : Coronavirus OC43, Human, Nucleocapsid Proteins, Recombinant Proteins.
- methods : Blotting, Western, Clinical Laboratory Techniques.
- Adolescent, Adult, Aged, Aged, 80 and over, Animals, Female, Humans, Male, Middle Aged, Young Adult.
Abstract
► We detected antibodies to HCoV-OC43 NP in over 90% of tested cord blood samples. ► Three patterns for the immunoreactivities of the structural regions of HCoV-OC43 NP. ► The central-linker region of the NP showed highly immunoreactive in human serum.
Url:
DOI: 10.1016/j.jviromet.2012.11.009
PubMed: 23174159
PubMed Central: 7112824
Affiliations:
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Pathology, Tri-Service General Hospital, National Defense University, Taipei, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Pathology, Tri-Service General Hospital, National Defense University, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Tang, Tswen Kei" sort="Tang, Tswen Kei" uniqKey="Tang T" first="Tswen-Kei" last="Tang">Tswen-Kei Tang</name><affiliation wicri:level="1"><nlm:aff id="aff0035">Department of Medical Laboratory Science and Biotechnology, Yuanpei University, Hsinchu, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Medical Laboratory Science and Biotechnology, Yuanpei University, Hsinchu</wicri:regionArea><wicri:noRegion>Hsinchu</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Yi Wen" sort="Chen, Yi Wen" uniqKey="Chen Y" first="Yi-Wen" last="Chen">Yi-Wen Chen</name><affiliation wicri:level="1"><nlm:aff id="aff0030">Department of Pathology, Tri-Service General Hospital, National Defense University, Taipei, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Pathology, Tri-Service General Hospital, National Defense University, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Hou, Ming Hon" sort="Hou, Ming Hon" uniqKey="Hou M" first="Ming-Hon" last="Hou">Ming-Hon Hou</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Biotechnology Center, National Chung Hsing University, Taichung, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Biotechnology Center, National Chung Hsing University, Taichung</wicri:regionArea><wicri:noRegion>Taichung</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0040">College of Life Science, National Chung Hsing University, Taichung, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>College of Life Science, National Chung Hsing University, Taichung</wicri:regionArea><wicri:noRegion>Taichung</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0045">Institute of Genomics and Bioinformatics, National Chung Hsing University, Taichung, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Genomics and Bioinformatics, National Chung Hsing University, Taichung</wicri:regionArea><wicri:noRegion>Taichung</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of Virological Methods</title><idno type="ISSN">0166-0934</idno><idno type="eISSN">1879-0984</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Animals</term><term>Antibodies, Viral (blood)</term><term>Blotting, Western (methods)</term><term>Clinical Laboratory Techniques (methods)</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus OC43, Human (immunology)</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Nucleocapsid Proteins (immunology)</term><term>Recombinant Proteins (immunology)</term><term>Young Adult</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Coronavirus humain OC43 (immunologie)</term><term>Femelle</term><term>Humains</term><term>Infections à coronavirus (diagnostic)</term><term>Jeune adulte</term><term>Mâle</term><term>Protéines nucléocapside (immunologie)</term><term>Protéines recombinantes (immunologie)</term><term>Sujet âgé</term><term>Sujet âgé de 80 ans ou plus</term><term>Technique de Western ()</term><term>Techniques de laboratoire clinique ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Coronavirus humain OC43</term><term>Protéines nucléocapside</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronavirus OC43, Human</term><term>Nucleocapsid Proteins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Blotting, Western</term><term>Clinical Laboratory Techniques</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Animals</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Young Adult</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Animaux</term><term>Femelle</term><term>Humains</term><term>Jeune adulte</term><term>Mâle</term><term>Sujet âgé</term><term>Sujet âgé de 80 ans ou plus</term><term>Technique de Western</term><term>Techniques de laboratoire clinique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Highlights</title><p>► We detected antibodies to HCoV-OC43 NP in over 90% of tested cord blood samples. ► Three patterns for the immunoreactivities of the structural regions of HCoV-OC43 NP. ► The central-linker region of the NP showed highly immunoreactive in human serum.</p></div></front><back><div1 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Gao</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Taïwan</li></country></list><tree><country name="Taïwan"><noRegion><name sortKey="Liang, Fang Ying" sort="Liang, Fang Ying" uniqKey="Liang F" first="Fang-Ying" last="Liang">Fang-Ying Liang</name></noRegion><name sortKey="Chen, Yi Wen" sort="Chen, Yi Wen" uniqKey="Chen Y" first="Yi-Wen" last="Chen">Yi-Wen Chen</name><name sortKey="Hou, Ming Hon" sort="Hou, Ming Hon" uniqKey="Hou M" first="Ming-Hon" last="Hou">Ming-Hon Hou</name><name sortKey="Hou, Ming Hon" sort="Hou, Ming Hon" uniqKey="Hou M" first="Ming-Hon" last="Hou">Ming-Hon Hou</name><name sortKey="Hou, Ming Hon" sort="Hou, Ming Hon" uniqKey="Hou M" first="Ming-Hon" last="Hou">Ming-Hon Hou</name><name sortKey="Liang, Fang Ying" sort="Liang, Fang Ying" uniqKey="Liang F" first="Fang-Ying" last="Liang">Fang-Ying Liang</name><name sortKey="Lin, Leng Chieh" sort="Lin, Leng Chieh" uniqKey="Lin L" first="Leng-Chieh" last="Lin">Leng-Chieh Lin</name><name sortKey="Lin, Leng Chieh" sort="Lin, Leng Chieh" uniqKey="Lin L" first="Leng-Chieh" last="Lin">Leng-Chieh Lin</name><name sortKey="Tang, Tswen Kei" sort="Tang, Tswen Kei" uniqKey="Tang T" first="Tswen-Kei" last="Tang">Tswen-Kei Tang</name><name sortKey="Yao, Chen Wen" sort="Yao, Chen Wen" uniqKey="Yao C" first="Chen-Wen" last="Yao">Chen-Wen Yao</name><name sortKey="Ying, Tsung Ho" sort="Ying, Tsung Ho" uniqKey="Ying T" first="Tsung-Ho" last="Ying">Tsung-Ho Ying</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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